山東大學(xué)生命科學(xué)學(xué)院導(dǎo)師：王金星 正文

　　王金星 教授
　　博士生導(dǎo)師
　　濟(jì)南市山東大學(xué)生命科學(xué)學(xué)院
　　jxwang@sdu.edu.cn
　　
　　教育背景
　　男，1955年5月生，博士，教授。2000年被聘為博士生導(dǎo)師
　　中國(guó)動(dòng)物學(xué)會(huì)理事，中國(guó)海洋與湖沼學(xué)會(huì)理事，山東動(dòng)物學(xué)會(huì)常務(wù)副理事長(zhǎng)，山東生化與分子生物學(xué)會(huì)副理事長(zhǎng)。享受?chē)?guó)務(wù)院政府特殊津貼。
　　1981年山東師范大學(xué)本科畢業(yè)、1984年山東大學(xué)碩士研究生畢業(yè)，留校工作。1998年獲博士學(xué)位。曾分別赴日本山口大學(xué)（1992）、九州工業(yè)大學(xué)（2000）、德國(guó)吉森大學(xué)（2002）等進(jìn)行合作研究。
　　培養(yǎng)的博士生有1人獲山東省優(yōu)秀博士論文，多人獲山東大學(xué)校長(zhǎng)獎(jiǎng)學(xué)金。研究生畢業(yè)后適合從事動(dòng)物學(xué)、水產(chǎn)科學(xué)、生化與分子生物學(xué)和生物工程等方面的教學(xué)與科研工作。
　　
　　研究方向
　　研究領(lǐng)域?yàn)閯?dòng)物學(xué)、生化與分子生物學(xué)。研究方向?yàn)闊o(wú)脊椎動(dòng)物先天免疫，包括先天免疫的模式識(shí)別、信號(hào)轉(zhuǎn)導(dǎo)途徑和效應(yīng)分子(主要是抗菌肽)及其功能。講授無(wú)脊椎動(dòng)物天然免疫等課程。
　　近十幾年來(lái)一直從事無(wú)脊椎動(dòng)物先天免疫研究。主持和參加國(guó)家自然科學(xué)基金、國(guó)家863、973、國(guó)家攻關(guān)和省部級(jí)等項(xiàng)目20余項(xiàng)。在The Journal of Immunology, Cellular and Molecular Life Sciences，Molecular Immunology, Developmental and Comparative immunology等國(guó)內(nèi)外刊物發(fā)表論文100余篇。獲國(guó)家發(fā)明專(zhuān)利5項(xiàng)。近年來(lái)在無(wú)脊椎動(dòng)物先天免疫研究方面取得了較好的進(jìn)展，獲教育部2010年高等學(xué)校科學(xué)研究?jī)?yōu)秀成果獎(jiǎng)—自然科學(xué)一等獎(jiǎng)。 主要在下列幾個(gè)方面開(kāi)展了較系統(tǒng)的工作。
　　1．感染的模式識(shí)別研究：多種免疫識(shí)別受體的發(fā)現(xiàn)，改變了先天免疫的早期的概念，先天免疫可以通過(guò)模式識(shí)別受體(PRRs)識(shí)別不同的病原相關(guān)分子模式(PAMPs)，而實(shí)現(xiàn)對(duì)不同病原的識(shí)別，因而存在著某種特異性。我們以對(duì)蝦為材料，發(fā)現(xiàn)了多種模式識(shí)別受體分子，對(duì)它們的性質(zhì)和功能進(jìn)行了較系統(tǒng)的研究。包括革蘭氏陰性菌結(jié)合蛋白（Molecular Immunology，2007）、6種C-型凝集素（Molecular Immunology，2008, 2009，Developmental & Comparative Immunology, 2009; Fish & Shellfish Immunology,2009）等，發(fā)現(xiàn)它們具有結(jié)合不同病原菌或不同病原相關(guān)分子模式的功能。并提出對(duì)蝦圍食膜蛋白(Molecular Immunology，2006)和血小板反應(yīng)素(Molecular Immunology，2006)可能與免疫有關(guān)，具有免疫識(shí)別的作用。
　　2．免疫信號(hào)的調(diào)整與信號(hào)轉(zhuǎn)導(dǎo)途徑：在模式生物研究中，發(fā)現(xiàn)了多種信號(hào)轉(zhuǎn)導(dǎo)途徑與動(dòng)物的先天免疫有關(guān)。包括在Toll樣途徑、免疫缺陷信號(hào)轉(zhuǎn)導(dǎo)途徑（Imd）途徑、Jak-Stat途徑、轉(zhuǎn)化生長(zhǎng)因子beta途徑、p38絲裂原活化的蛋白激酶途徑、細(xì)胞程序化死亡途徑和胰島素樣受體途徑等。我們近期的工作發(fā)現(xiàn)了多種可能參與免疫信號(hào)的調(diào)整的蛋白酶及其抑制因子，包括含有Clip結(jié)構(gòu)域的絲氨酸蛋白酶及其同系物(Fish & Shellfish Immunology, 2009)、絲氨酸蛋白酶抑制因子(Fish & Shellfish Immunol, 2009)。同樣發(fā)現(xiàn)了多種與免疫信號(hào)轉(zhuǎn)導(dǎo)相關(guān)的關(guān)鍵分子，包括Toll樣受體及其配體Sp?tzle (Fish & Shellfish Immunology,2009) 、IMD、STAT及多種磷酸激酶等相關(guān)的分子。初步實(shí)驗(yàn)結(jié)果顯示Toll途徑可能參與Crustin 抗菌肽表達(dá)的調(diào)控。
　　3．免疫效應(yīng)分子的研究：無(wú)脊椎動(dòng)物主要的免疫效應(yīng)分子為抗菌肽（蛋白）。研究了中國(guó)對(duì)蝦和家蠅的多種抗菌肽的性質(zhì)、功能與表達(dá)調(diào)控，并成功進(jìn)行了原核和真核重組表達(dá)。首先對(duì)家蠅的抗菌肽進(jìn)行研究，克隆到了多種家蠅抗菌肽（包括天蠶素、防御素、攻擊素和溶菌酶等）基因，對(duì)其時(shí)空表達(dá)與調(diào)控進(jìn)行了研究，并進(jìn)行了原核和/或真核重組表達(dá)，獲得了有抗菌活性的重組抗菌肽（Developmental & Comparative Immunology 2006， Cellular and Molecular Life Sciences 2006）。對(duì)蝦抗菌肽研究： 對(duì)中國(guó)明對(duì)蝦抗菌肽——對(duì)蝦素基因進(jìn)行了克隆與表達(dá)調(diào)控研究，得到2類(lèi)（共4種）對(duì)蝦素基因，在對(duì)蝦中首次發(fā)現(xiàn)和命名對(duì)蝦素5，在大腸桿菌和酵母菌中成功表達(dá)了兩類(lèi)對(duì)蝦素這些抗菌肽均顯示出廣譜抗菌活性，具有潛在的應(yīng)用前景(Fish & Shellfish Immunology 2004；Protein expression and purification 2004；Molecular Immunology，2007)。發(fā)現(xiàn)了多種含有WAP結(jié)構(gòu)域的抗菌肽，包括SWD（Aquaculture，2008）、甲殼肽（Fish & Shellfish Immunology，2010）、抗脂多糖因子、溶菌酶等，并進(jìn)行了重組表達(dá)的性質(zhì)研究，得到了有抗菌活性的上述分子。
　　4．抗病毒機(jī)理的研究：RNA干擾途徑在無(wú)脊椎動(dòng)物中具有重要的抗病毒功能。我們?cè)谥袊?guó)明對(duì)蝦中發(fā)現(xiàn)了轉(zhuǎn)錄激活反應(yīng)RNA結(jié)合蛋白(TRBP)與真核翻譯起始因子6(eIF6)之間存在著相互作用，可能與是RNAi途徑的重要組成成分(Journal of Immunology, 2009)。另外還發(fā)現(xiàn)了多種可能參與抗病毒反應(yīng)的蛋白(Molecular Biology Reports，2009)。
　　
　　承擔(dān)課題
　　在研項(xiàng)目包括973 、863、國(guó)家自然科學(xué)基金、海外青年合作研究基金（杰出青年基金B(yǎng)）和高校博士點(diǎn)基金等。
　　
　　研究成果及發(fā)表論文
　　代表性論著 (* 通訊作者Corresponding author):
　　54. Chen AJ, Wang S, Zhao XF, Yu XQ, Wang JX*. E2 Enzyme from Chinese White Shrimp Inhibits Replication of White Spot Syndrome Virus and Ubiquitinates its RING Domain Proteins. J. Virol. 2011, doi:10.1128/JVI.00487-11.
　　53. Ren Q, Zhou J, Zhao XF, Wang JX*. Potential role of single hotdog fold thioesterase in the antiviral response of Fenneropenaeus chinensis. Fish Shellfish Immunol. 2011 Feb 26. doi:10.1016/j.fsi.2011.02.019
　　52. Zhang XW, Wang XW, Sun C, Zhao XF, Wang JX*. C-type lectin from red swamp crayfish Procambarus clarkii participates in cellular immune response. Arch Insect Biochem Physiol. 2011 Mar;76(3):168-84.
　　51. Sun C, Shao HL, Zhang XW, Zhao XF, Wang JX*. Molecular cloning and expression analysis of signal transducer and activator of transcription (STAT) from the Chinese white shrimp Fenneropenaeus chinensis. Mol Biol Rep. 2011 Jan 19. DOI 10.1007/s11033-011-0681-x
　　50. Ren Q, Zhou J, Zhao XF, Wang JX*. Molecular cloning and characterization of a receptor for activated protein kinase C1 (RACK1) from Chinese white shrimp; Fenneropenaeus chinensis. Dev Comp Immunol. 2011 Jan 14. doi:10.1016/j.dci.2011.01.004
　　49. Ren Q, Zhao XF, Wang JX*. Identification of three different types of serine proteases (one SP and two SPHs) in Chinese white shrimp. Fish Shellfish Immunol. 2011 Feb; 30(2):456-66. doi:10.1016/j.fsi.2010.11.009
　　48. Ren Q, Zhou J, Sun SS, Kang CJ, Zhao XF, Wang JX*. Molecular cloning and expression pattern analysis of two novel disulfide isomerases in shrimp. Comp Biochem Physiol C Toxicol Pharmacol. 2011 Apr;153(3):301-9. doi:10.1016/j.cbpc.2010.11.007
　　47. Sun C, Xu WT, Zhang HW, Dong LP, Zhang T, Zhao XF, Wang JX* An anti-Lipopolysaccharide factor from red swamp crayfish, Procambarus Clarkii, exhibited antimicrobial activities in vitro and in vivo. Fish Shellfish Immunol. 2011 Jan;30(1):295-303. doi:10.1016/j.fsi.2010.10.022
　　46. Wang XW, Zhang HW, Li X, Zhao XF, Wang JX*. Characterization of a C-type lectin (Pclec2) as an upstream detector in the prophenoloxidase activating system of red swamp crayfish. Fish Shellfish Immunol. 2011 Jan; 30(1):241-7. doi:10.1016/j.fsi.2010.10.012
　　45. Zhang HW, Sun C, Sun SS, Zhao XF, Wang JX* Functional analysis of two invertebrate-type lysozymes from red swamp crayfish, Procambarus clarkii. Fish Shellfish Immunol. 2010 Dec; 29(6):1066-72. Doi: 10.1016/j.fsi.2010.08.023
　　44. Chai YM Yu SS, Zhao XF, Zhu Q, Wang JX*, Comparative proteomic profiles of the hepatopancreasin Fenneropenaeus chinensis response to white spot syndrome virus. Fish Shellfish Immunol. 2010 Sep;29(3): 480-6. doi:10.1016/j.fsi.2010.05.009
　　43. Ren Q, Zhang XW, Sun YD, Sun SS, Zhou J, Zhao XF, Wang JX*. Two Cysteine proteinases respond to bacterial and WSSV challenge in Chinese white shrimp Fenneropenaeus chinensis. Fish Shellfish Immunol. 2010 Oct;29(4):551-6. DOI: 10.1016/ j.fsi.2010.03.002
　　42. Wang Q, Liu Y, He H-J, Zhao X-F*, Wang J-X*. Immune responses of Helicoverpa armigera to different kinds of pathogens. BMC Immunology, 2010, BMC Immunol. 2010 Mar 3;11:9.
　　41. Xu W-T, Wang X-W, Zhang X-W, Zhao X-F, Yu X-Q, Wang J-X*. A new C-type lectin (FcLec5) from the Chinese white shrimp Fenneropenaeus chinensis. Amino Acids, 2010 Nov;39(5):1227-39. DOI 10.1007/s00726-010-0558-72010.
　　40. Li X-C, Zhang R-R, Sun R-R, Lan J-F, Zhao X-F, Wang J-X*. Three Kazal-type serine proteinase inhibitors from the red swamp crayfish Procambarus clarkii and the characterization, function analysis of hcPcSPI2. Fish Shellfish Immunol. 2010 May-Jun; 28(5-6):942-951.
　　39. Shi X-Z, Li X-C, Wang S, Zhao X-F, Wang J-X*. Transcriptome analysis of hemocytes and hepatopancreas in red swamp crayfish, Procambarus clarkii, challenged with white spot syndrome virus. Invertebrate Survival Journal, 2010, Apr; 7: 119-131.
　　38. Sun C, Du XJ, Xu WT, Zhang HW, Zhao XF, Wang JX*. Molecular cloning and characterization of three crustins from the Chinese white shrimp, Fenneropenaeus chinensis. Fish Shellfish Immunol. 2010 Apr; 28(4):517-24.
　　37. Ren Q, Zhang RR, Zhao XF, Wang JX*. A thioredoxin response to the WSSV challenge on the Chinese white shrimp, Fenneropenaeus chinensis. Comp Biochem Physiol C Toxicol Pharmacol. 2010 Jan;151(1):92-98.
　　36. Du ZQ, Li XC, Wang ZH, Zhao XF, Wang JX*. A single WAP domain (SWD)-containing protein with antipathogenic relevance in red swamp crayfish, Procambarus clarkii. Fish Shellfish Immunol. 2010; 28(1): 134-142
　　35. Shi XZ, Zhang RR, Jia YP, Zhao XF, Yu XQ*, Wang JX*. Identification and molecular characterization of a Sp?tzle-like protein from Chinese shrimp (Fenneropenaeus chinensis). Fish Shellfish Immunol. 2009;27(5):610-7.
　　34. Ren Q, Du ZQ, Zhao XF, Wang JX*. An acyl-CoA-binding protein (FcACBP) and a fatty acid binding protein (FcFABP) respond to microbial infection in Chinese white shrimp, Fenneropenaeus chinensis. Fish Shellfish Immunol. 2009; 27: 739-747.
　　33. Wang XW, Xu WT, Zhang XW, Zhao XF, Yu XQ*, Wang JX*. A C-type lectin is involved in the innate immune response of Chinese white shrimp. Fish Shellfish Immunol. 2009 Sep, 27(4): 556-562.
　　32. Li XC, Wang XW, Wang ZH, Zhao XF, Wang JX*. A three-domain Kazal-type serine proteinase inhibitor exhibiting domain inhibitory and bacteriostatic activities from freshwater crayfish Procambarus clarkii. Dev Comp Immunol. 2009 Dec; 33(12): 1229-1238.
　　31. Du ZQ, Ren Q, Zhao XF, Wang JX*. A double WAP domain (DWD)-containing protein with proteinase inhibitory activity in Chinese white shrimp, Fenneropenaeus chinensis.Comp Biochem Physiol B Biochem Mol Biol. 2009 Oct;154(2):203-10.
　　30. Wang XW, Zhang X-W, Xu W-T, Zhao X-F, Wang J-X*. A novel C-type lectin (FcLec4) facilitates the clearance of Vibrio anguillarum in vivo in Chinese white shrimp. Dev Comp Immunol. 2009 Sep;33(9):1039-47.
　　29. Wang ZH, Zhao XF, Wang JX*. Characterization, kinetics, and possible function of Kazal-type proteinase inhibitors of Chinese white shrimp, Fenneropenaeus chinensis. Fish Shellfish Immunol. 2009 Jun; 26(6): 885-897.
　　28. Wang S , Liu N, Chen AJ, Zhao XF, Wang JX*. TRBP homologue interacts with Eukaryotic Initiation Factor 6 (eIF6) in Fenneropenaeus chinensis. J Immunol. 2009 May 1; 182(9):5250-5258.
　　27. Ren Q, Xu ZL, Wang XW Zhao XF, Wang JX*. Clip domain serine protease and its homolog respond to Vibrio challenge in Chinese white shrimp, Fenneropenaeus chinensis. Fish Shellfish Immunol., 2009 May; 26(5):787-798.
　　26. Zhang XW, Xu WT, Wang XW, Mu Y, Zhao XF, Yu XQ*, Wang JX*. A novel C-type lectin with two CRD domains from Chinese shrimp Fenneropenaeus chinensis functions as a pattern recognition protein. Mol. Immunol., 2009 May; 46(8-9): 1626-1637.
　　25. Shi XZ, Ren Q, Zhao XF, Wang JX*. Expression of four trypsins from the Chinese shrimp, Fenneropenaeus chinensis, as regulated by pathogenic infection. Comp Biochem Physiol. B 2009; 153: 54–60.
　　24. Ren Q, Sun RR, Zhao XF, Wang JX*. A selenium-dependent glutathione peroxidase (Se-GPx) and two glutathione S-transferases (GSTs) from Fenneropenaeus chinensis, Chinese shrimp. Comp Biochem Physiol. C. Comp Biochem Physiol C Toxicol Pharmacol. 2009 May; 149(4):613-23.
　　23. Tian YY, Liu Y, Zhao XF*, Wang JX*. Characterization of a C-type lectin from the cotton bollworm, Helicoverpa armigera. Dev. Comp. Immunol. 2009; 33: 772-779.
　　22. Ren Q, Zhao XF, Wang JX*. Molecular characterization and expression analysis of a chicken-type lysozyme gene from housefly Musca domestica. J Genet Genomics 2009; 36: 7-16.
　　21. Wang S, Zhao XF, Wang JX*. Molecular cloning and characterization of the translationally controlled tumor protein from Fenneropenaeus chinensis. Mol Biol Rep. 2009 Sep;36(7):1683-93. Epub 2008 Oct 14.
　　20. Zhao XF, Wang JX*. The antimicrobial peptides of shrimp immune response. Invertebrate Survival Journal 2008; 5: 162-179.
　　19. Jia YP Yu, Sun YD, Wang ZH, Wang Q, Wang XW, Zhao XF, Wang JX*. A single whey acidic protein domain (SWD)-containing peptide from fleshy prawn with antimicrobial and proteinase inhibitory activities. Aquaculture 2008; 284:246-259.
　　18. Shi XZ, Zhao XF, Wang JX*. Molecular cloning and expression analysis of chymotrypsin-like serine protease from the Chinese shrimp, Fenneropenaeus chinensis. Fish Shellfish Immunol. 2008; 25 589-597.
　　17. Bu XJ, Du XJ, Zhou WJ, Zhao XF, Wang JX*. Molecular cloning, recombinant expression, and characterization of lysozyme from Chinese shrimp Fenneropenaeus chinensis. Chinese J. Biotechnol., 2008; 24:723-732.
　　16. Ren Qian, Zhang Wei, Zhao Xiao-Fan, Wang Jin-Xing*. Gene cloning and expression analysis of ubiquitin derived from Musca domestica. Arch Insect Biochem Physiol. 2008; 68(2):89-99.
　　15. Shi XZ, Zhao X-F, and Wang J-X*. Molecular Cloning and Analysis of Function of Nucleoside Diphosphate Kinase (NDPK) from the Scallop Chlamys farreri. Biochemistry (Moscow), 2008; 73:686-692.
　　14. Sun YD, Fu LD, Jia YP, Du XJ, Wang Q, Wang YH, Zhao XF, Yu XQ, Wang JX*. A hepatopancreas-specific C-type lectin from the Chinese shrimp Fenneropenaeus chinensis exhibits antimicrobial activity. Mol Immunol. 2008; 45(2):348-361.
　　13. Chai LQ, Tian YY, Wang JX*, Zhao XF*. Molecular cloning and expression analysis of a C-type lectin from the cotton bollworm, Helicoverpa armigera. Dev Comp Immunol. 2008;32(1):71-83.
　　12. Wang JX*, Zhao XF, Liang YL, Li L, Zhang W, Ren Q, Wang LC, Wang LY. Molecular characterization and expression of the antimicrobial peptide defensin from the housefly (Musca domestica). Cell Mol. Life Sci. 2006; 63(24):3072-3082.
　　11. Kang CJ, Xue JF, Liu N, Zhao XF, Wang JX*. Characterization and expression of a new subfamily member of penaeidin antimicrobial peptides (penaeidin 5) from Fenneropenaeus chinensis. Mol. Immunol. 2007; 44(7):1535-1543.
　　10. Du XJ, Zhao XF, Wang JX*. Molecular cloning and characterization of a lipopolysaccharide and beta-1,3-glucan binding protein from fleshy prawn (Fenneropenaeus chinensis).Mol Immunol. 2007; 44: 1085-1094.
　　9. Liang YL, Wang JX*, Zhao XF, Du XJ, Xue JF. Molecular cloning and characterization of cecropin from the housefly (Musca domestica), and its expression in Escherichia coli.Dev Comp Immunol. 2006; 30: 249-257.
　　8. Sun YD, Zhao XF, Kang CJ, Wang JX*. Molecular cloning and characterization of Fc-TSP from the Chinese shrimp Fenneropenaeus chinensis. Mol Immunol. 2006; 43: 1202-1210.
　　7. Du XJ, Wang JX*, Liu N, Zhao XF, Li FH, Xiang JH. Identification and molecular characterization of a peritrophin-like protein from fleshy prawn (Fenneropenaeus chinensis). Mol Immunol. 2006; 43: 1633-1644.
　　6. Li DX, Du XJ, Zhao XF, Wang JX*. Cloning and expression analysis of an o-methyltransferase (OMT) gene from Chinese shrimp, Fenneropenaeus chinensis. Fish Shellfish Immunol. 2006; 21(3):284-292.
　　5. Li L, Wang JX*, Zhao XF, Kang CJ, Liu N, et al. High level expression, purification, and characterization of the shrimp antimicrobial peptide, Ch-penaeidin, in Pichia pastoris. Protein Expr Purif. 2005; 39 (2):144-151.
　　4. Wang JX*, Zhao XF. Progress in pattern recognition receptors of innate immunity in invertebrate. Prog. Biochem. Biophys. 2004; 31: 112-117.
　　3. Kang CJ, Wang JX*, Zhao XF, Yang XM, Shao HL, Xiang JH. Molecular cloning and expression analysis of Ch-penaeidin, an antimicrobial peptide from Chinese shrimp, Fenneropenaeus chinese. Fish Shellfish Immunol. 2004; 16:513-525.
　　2. Wang J-X*, Zhao XF, Deng Y, Qi HY, Wang ZJ. Chromosomal polymorphism of mandarin vole, Microtus mandarinus (Rodentia). Hereditas. 2003;138(1):47-53.
　　1. Wang JX*, Zhao XF, Koh HS, Deng Y, Qi HY. Chromosomal polymorphisms due to heterochromatin growth and pericentric inversions in white-bellied rat, Niviventer confucianus, from China. Hereditas. 2003;138(1):59-64.

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